ETHOS™ X For Fat Determination

Microwave Fat Determination in Food and Feed

Fat determination in food and feed matrices is a routine analysis that still relies on outdated and matrix-dependent methods which use obsolete and time-consuming technologies. The ETHOS X offers a new approach to gravimetric fat determination, moving toward a more modern, greener and more efficient approach to total fat, Fatty Acid Methyl Ester (FAME) and free fat determinations in food and feed matrices.

Gain Productivity in Fat Determination on any Matrix

Total fat determination in food and feed matrices is a routine analysis for several testing and food processor labs, as it is part of labelling process and QC testing. Nowadays, the process still relies on outdated and matrix-dependent methods which use obsolete and time-consuming technologies.
In the ETHOS X hydrolysis and extraction are done in a single step and in less time, ensuring higher productivity and better workflow.
The ETHOS X method for total fat determination is applicable to any matrix; it enables to easily process 45 sample a day, ensures lower cost per sample, offers complete fat determination sand it is safe and compact.
  • Applicable to any matrix
  • 45 samples a day
  • Lower cost per sample
  • Complete fat determination
  • Safe and compact


The traditional fat extraction methods are very labour intensive and time-consuming.
Compare the performances of the ETHOS X microwave-based technology to those of the Reference Method

Conventional total fat determination methods, such as Soxhlet and automated Soxhlet, go through a multi-step process, that includes long hydrolysis and extraction procedures, while secondary methods are matrix-dependent and require frequent calibrations. The ETHOS X allows to process any food and feed matrix with a single approach, directly impacting the lab workflow.

In addition to the commonly performed total fat determination, the ETHOS X makes FAME and free fat feasible within a single platform. Unlike Soxhlet and automated Soxhlet, the ETHOS X is not installed in the fume hood and saves over 50% of the lab space. Its dedicated exhaust system, the rugged hardware construction, the efficient temperature control, and the up-to-date software features ensure highest operator safety level throughout the entire process.

Improved Workflow & Productivity

In QC and food testing laboratories a fast delivery of results and high throughput are key factors for total fat determination. Even modern automated Soxhlet techniques require several hours in order to deliver results affecting the competitiveness of the lab too. The ETHOS X processes 15 samples simultaneously, and as many as 45 samples in a single working day, using one rotor and 75 samples with two rotors. Its specific method combines the hydrolysis and extraction into a single step, thus having a direct impact on the lab’s turnaround time and throughput.

Lower cost per sample

Traditional extraction techniques are based on the Soxhlet technique, which uses elevated acid and solvent volumes for the hydrolysis and extraction steps, respectively. The ETHOS X offers a substantial reagent and waste reduction, thanks to the use of the closed-vessel technology. While Soxhlet and automated Soxhlet systems demand around 100/ 200 mL of reagents per sample, the ETHOS X only requires 35 mL. The low reagent consumption in combination with the higher productivity and competitive initial investment offers lower cost per sample.


In addition to total fat determination, Fatty Acid Methyl Ester (FAME) analysis is a common analysis as it determines saturated and unsaturated fats. The flexibility of the ETHOS X configuration and its dedicated procedures enables to perform FAME and free fat determinations of food samples, performing the complete fatty acids profile within a single platform.
Saturated fat Monounsaturated Polyunsaturated
Reference value 14.40 15.40 6.40
ETHOS X (%) 16.29 14.53 6.18
Saturated fat Monounsaturated Polyunsaturated
Reference value 0.27 0.37 0.98
ETHOS X (%) 0.30 0.39 0.94
Saturated fat Monounsaturated Polyunsaturated
Reference value 10.82 30.34 58.85
ETHOS X (%) 10.88 30.57 58.55
Complete fat profiles on three samples using ETHOS X methods